8ZIK

Crystal structure of a beta-1,4-endoglucanase from Bispora sp. MEY-1 in complex with cellotetraose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	193
Detector technology	PIXEL
Collection date	2020-01-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
Unit cell lengths	72.318, 94.990, 179.759
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.860 - 1.870
R-factor	0.1908
Rwork	0.188
R-free	0.23910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.967
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.860	10.000	1.900
High resolution limit [Å]	1.870	5.070	1.870
Rmerge	0.130	0.067	0.859
Rmeas	0.136	0.070	0.945
Rpim	0.040	0.020	0.380
Number of reflections	92829	5489	3223
<I/σ(I)>	6.7
Completeness [%]	90.2	100	63.3
Redundancy	10.3	12.3	4.5
CC(1/2)	0.991	0.998	0.694

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1 M Tris, pH 7.5, 0.2 M sodium acetate, 32% PEG 4000