8ZII
Crystal structure of the methyltransferase PsiM in complex with SAH and Norbaeocystin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 39.422, 57.530, 129.465 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.110 - 2.500 |
| R-factor | 0.1759 |
| Rwork | 0.174 |
| R-free | 0.21730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8zie |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.991 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.186 | 0.530 |
| Rmeas | 0.194 | 0.553 |
| Rpim | 0.054 | 0.154 |
| Number of reflections | 10761 | 1527 |
| <I/σ(I)> | 10.9 | 4.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 12.6 | 12.7 |
| CC(1/2) | 0.995 | 0.964 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 40% (v/v) Isopropanol,0.1M Imidazole/Hydrochloric acid pH 6.5, 15% (w/v) PEG 8000 |
