8ZHZ

Structure of Ikoma lyssavirus glycoprotein in pre-fusion state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97930
Spacegroup name	P 1 21 1
Unit cell lengths	122.915, 90.746, 151.311
Unit cell angles	90.00, 90.08, 90.00

Refinement procedure

Resolution	46.640 - 2.880
R-factor	0.2278
Rwork	0.225
R-free	0.28120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.348
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.880	2.880
Number of reflections	74555	7419
<I/σ(I)>	9.15
Completeness [%]	99.6
Redundancy	5.1
CC(1/2)	0.969	0.659

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289	0.2M Ammonium phosphate dibasic (pH 8.3), 20% w/v Polyethylene glycol 3350