8ZHW

Structure of Mokola lyssavirus glycoprotein in post-fusion state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-11
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97915
Spacegroup name	P 1 2 1
Unit cell lengths	104.235, 87.518, 111.189
Unit cell angles	90.00, 111.87, 90.00

Refinement procedure

Resolution	48.366 - 3.199
R-factor	0.2484
Rwork	0.246
R-free	0.28770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.750
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.310
High resolution limit [Å]	3.199	3.199
Number of reflections	29464	5304
<I/σ(I)>	14.58
Completeness [%]	95.2
Redundancy	7.2
CC(1/2)	0.999	0.640

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	0.1M Sodium acetate trihydrate (pH 4.0), 10% w/v Polyethylene glycol 4000