8ZHT

Structure of PpiD-YfgM complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL18U
Synchrotron site	NFPSS
Beamline	BL18U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-08-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	P 2 21 21
Unit cell lengths	76.566, 81.347, 212.851
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.190 - 3.000
R-factor	0.1818
Rwork	0.181
R-free	0.21810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.848
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.190	2.279
High resolution limit [Å]	2.200	2.200
Number of reflections	65423	65423
<I/σ(I)>	1.49
Completeness [%]	95.6
Redundancy	9.9
CC(1/2)	0.980	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	PEG3350, Sodium citrate, n-Octyl-D-glucopyranoside