8ZGU

Crystal structural of HTNV Gn and AH100 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-06-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00389
Spacegroup name	I 4 2 2
Unit cell lengths	170.380, 170.380, 117.114
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.260 - 3.510
R-factor	0.2548
Rwork	0.253
R-free	0.29500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.706
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.720
High resolution limit [Å]	3.510	3.510
Number of reflections	20660	10
<I/σ(I)>	14.7	2.1
Completeness [%]	99.9
Redundancy	13.9
CC(1/2)	0.999	0.856

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M sodium citrate, pH5.5, 50% PEG 6000