8ZGA

F-degron fused ZZ-domain of the Arabidopsis thaliana E3 ubiquitin-protein ligase PRT1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.28
Spacegroup name	P 21 21 21
Unit cell lengths	48.729, 86.439, 89.769
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.830 - 2.100
R-factor	0.2605
Rwork	0.257
R-free	0.29450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8zg8
RMSD bond length	0.004
RMSD bond angle	0.643
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18rc7_3834)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.830	2.175
High resolution limit [Å]	2.100	2.100
Rmerge	0.174	2.578
Rmeas	0.181	2.675
Rpim	0.049	0.705
Number of reflections	22752	2193
<I/σ(I)>	11.57	1.2
Completeness [%]	99.7
Redundancy	14
CC(1/2)	0.995	0.709

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Divalents Buffer System 2 pH 7.5 Precipitant Mix 4