8ZG9

Y-degron fused ZZ-domain of the Arabidopsis thaliana E3 ubiquitin-protein ligase PRT1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.28
Spacegroup name	P 21 21 21
Unit cell lengths	48.254, 85.737, 85.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.980 - 1.673
R-factor	0.212
Rwork	0.211
R-free	0.23680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8zg8
RMSD bond length	0.007
RMSD bond angle	0.995
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18rc7_3834)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.980	1.710
High resolution limit [Å]	1.673	1.680
Rmerge	0.160	1.147
Number of reflections	41405	3911
<I/σ(I)>	20.1
Completeness [%]	99.0
Redundancy	6.8
CC(1/2)		0.599

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Divalents Buffer System 2 pH 7.5 Precipitant Mix 4