8ZG5

Drimenyl diphosphate synthase SsDMS_Y505I&D303A from Streptomyces showdoensis (Apo)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-01-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	C 2 2 21
Unit cell lengths	51.450, 114.170, 180.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.410 - 1.830
R-factor	0.1829
Rwork	0.182
R-free	0.21140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.000
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.220	1.930
High resolution limit [Å]	1.830	1.830
Rmerge	0.061
Number of reflections	47399	6830
<I/σ(I)>	14.4
Completeness [%]	100.0
Redundancy	12.1
CC(1/2)	0.997	0.880

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289.15	HEPES, NaCl