8ZG1

Drimenyl diphosphate synthase SsDMS_F248Y&D303E from Streptomyces showdoensis (Apo)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-17
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	C 2 2 21
Unit cell lengths	51.580, 114.080, 181.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.260 - 2.400
R-factor	0.1994
Rwork	0.194
R-free	0.25040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.523
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.510	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.089
Number of reflections	20686	2925
<I/σ(I)>	9.7
Completeness [%]	96.8
Redundancy	6.3
CC(1/2)	0.990	0.861

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289.15	HEPES, NaCl