8ZG0

Drimenyl diphosphate synthase SsDMS_F248A&D303E from Streptomyces showdoensis (Apo)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-20
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	C 2 2 21
Unit cell lengths	51.390, 114.280, 180.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.600 - 1.980
R-factor	0.1787
Rwork	0.176
R-free	0.21870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7xq4
RMSD bond length	0.008
RMSD bond angle	0.932
Data reduction software	iMOSFLM (3.27)
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.460	2.090
High resolution limit [Å]	1.980	1.980
Rmerge	0.056
Number of reflections	37556	5430
<I/σ(I)>	18.9
Completeness [%]	99.8
Redundancy	11.1
CC(1/2)	0.995	0.988

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.9	289.15	HEPES, NaCl