8ZFV

Crystal Structure of C-terminal domain of nucleocapsid protein from SARS-CoV-2 in complex with ceftriaxone

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-24
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54184
Spacegroup name	P 1
Unit cell lengths	43.539, 46.643, 69.021
Unit cell angles	106.66, 90.11, 93.46

Refinement procedure

Resolution	26.580 - 2.000
R-factor	0.15019
Rwork	0.147
R-free	0.20494
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6yun
RMSD bond length	0.008
RMSD bond angle	1.844
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.580	2.050
High resolution limit [Å]	2.000	2.000
Number of reflections	34801	2572
<I/σ(I)>	17.8	3.8
Completeness [%]	99.2
Redundancy	4.2
CC(1/2)	0.997	0.889

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	30% PEG4000, 0.2 M Lithium sulfate, 50 mM Tris pH 8.0