8ZFH
Structure of the C-terminal domain of nsp4 from PDCOV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-01 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 61 |
| Unit cell lengths | 88.320, 88.320, 71.130 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.250 - 2.550 |
| R-factor | 0.2345 |
| Rwork | 0.231 |
| R-free | 0.29700 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.721 |
| Data reduction software | autoPROC |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.250 | 2.660 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.108 | 0.541 |
| Number of reflections | 10339 | 1250 |
| <I/σ(I)> | 22.5 | |
| Completeness [%] | 99.5 | |
| Redundancy | 19.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.0 M Sodium chloride,10% w/v Polyethylene glycol 6,000 |
