8ZF2

Quorum Sensing master regulator HapR of Photobacterium marinum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER X8 PROTEUM
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-14
Detector	Bruker PHOTON III
Wavelength(s)	1.54
Spacegroup name	P 1
Unit cell lengths	40.600, 44.310, 61.026
Unit cell angles	70.39, 72.78, 85.11

Refinement procedure

Resolution	26.040 - 1.800
R-factor	0.1903
Rwork	0.188
R-free	0.22890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7xxo
RMSD bond length	0.004
RMSD bond angle	0.655
Data reduction software	PROTEUM
Data scaling software	PROTEUM
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.130	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	35478	3559
<I/σ(I)>	17.4
Completeness [%]	100.0
Redundancy	8.8
CC(1/2)	0.999	0.701

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		295	20% PEG 3350, Sodium Citrate pH 4.0, 200 mM Sodium citrate tribasic