8ZEY

Anti-CRISPR type I subtype E3;AcrIE3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	125
Detector technology	PIXEL
Collection date	2024-03-22
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	H 3 2
Unit cell lengths	76.890, 76.890, 67.930
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.900 - 1.734
R-factor	0.2285
Rwork	0.228
R-free	0.23150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold
RMSD bond length	0.006
RMSD bond angle	0.958
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.900	1.796
High resolution limit [Å]	1.734	1.734
Rmerge	4.332
Number of reflections	8156	790
<I/σ(I)>	37.67
Completeness [%]	99.9
Redundancy	20
CC(1/2)		0.848

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	40% (w/v) PEG 400, 0.1M sodium cacodylate pH 6.5, 0.2M Calcium Acetate