8ZEF
Crystal structure of Staphylococcus aureus DinG protein in complex with ssDNA and Ca2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-01-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 68.855, 128.045, 302.999 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.650 - 3.210 |
| R-factor | 0.2836 |
| Rwork | 0.283 |
| R-free | 0.29170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.155 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.650 | 3.330 |
| High resolution limit [Å] | 3.210 | 3.210 |
| Rmerge | 0.102 | 1.514 |
| Number of reflections | 22275 | 3207 |
| <I/σ(I)> | 2.43 | 2.7 |
| Completeness [%] | 99.6 | |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 5% v/v Tacsimate (pH 7.0), 0.1 M HEPES (pH 7.0), 8% w/v Polyethylene glycol monomethyl ether 5,000 and 50 mM CaCl2. |
