Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 293.15 |
| Detector technology | PIXEL |
| Collection date | 2023-10-26 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.265, 92.917, 44.265 |
| Unit cell angles | 90.00, 90.18, 90.00 |
Refinement procedure
| Resolution | 29.710 - 1.650 |
| Rwork | 0.126 |
| R-free | 0.15840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6u9i |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.798 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.710 | 1.709 |
| High resolution limit [Å] | 1.600 | 1.650 |
| Number of reflections | 42230 | 4259 |
| <I/σ(I)> | 14.16 | |
| Completeness [%] | 99.2 | |
| Redundancy | 5.6 | |
| CC(1/2) | 0.998 | 0.815 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 2 M ammonium sulfate, 0.2 M potassium sodium tartrate tetrahydrate, and 0.1 M sodium citrate (pH 5.6) |
