8ZDE

Crystal structure of HsmR with DNA bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2023-07-19
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97179
Spacegroup name	C 1 2 1
Unit cell lengths	262.113, 55.705, 117.525
Unit cell angles	90.00, 101.35, 90.00

Refinement procedure

Resolution	50.000 - 2.910
R-factor	0.21573
Rwork	0.212
R-free	0.27599
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.453
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.900	2.900
Rmeas	0.133	1.093
Number of reflections	37178	1850
<I/σ(I)>	18.9
Completeness [%]	99.6
Redundancy	3.5
CC(1/2)	0.991	0.888

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1 M KCl, 35% PEP (5/4 PO/OH) and 50 mM HEPES (pH 7.5)