8ZD6
Crystal structure of E40K variant of Cu/Zn-superoxide dismutase from dog (Canis familiaris) in the apo form complexed with 22E1 Fv-clasp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.900 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.616, 83.601, 185.750 |
| Unit cell angles | 90.00, 94.52, 90.00 |
Refinement procedure
| Resolution | 43.940 - 3.290 |
| R-factor | 0.27938 |
| Rwork | 0.277 |
| R-free | 0.32642 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.978 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.830 | 3.420 |
| High resolution limit [Å] | 3.290 | 3.290 |
| Rpim | 0.055 | 0.847 |
| Number of reflections | 38902 | 4189 |
| <I/σ(I)> | 7.8 | 0.8 |
| Completeness [%] | 99.0 | |
| Redundancy | 3.4 | |
| CC(1/2) | 1.000 | 0.590 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 2% (v/v) polyethylene glycol 400, 0.1M HEPES sodium pH 7.5, 2.0M ammonium sulfate |
