8ZCM

Cryogenic Temperature Crystal Structure of Fc Fragment of Human IgG1 from Biosimilar VEGF-Trap

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	49.450, 79.620, 125.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.010 - 2.640
R-factor	0.3597
Rwork	0.354
R-free	0.40930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.547
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.010	7.920	2.800
High resolution limit [Å]	2.640	5.600	2.640
Rmerge	0.106	0.060	0.876
Rmeas	0.128	0.073	1.071
Number of reflections	14547	1009	2341
<I/σ(I)>	7.55
Completeness [%]	95.5
Redundancy	2.85
CC(1/2)	0.993	0.993	0.441

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		298	0.1M Bis-Tris pH 6.5, 25% w/v Polyethylene glycol 3350