8ZBO
Crystal structure of the biphotochromic fluorescent protein moxSAASoti (F97M variant) in its green on-state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-09 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.652, 81.254, 98.813 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.830 - 2.000 |
| R-factor | 0.18041 |
| Rwork | 0.178 |
| R-free | 0.23107 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.260 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.830 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.082 | 0.449 |
| Rmeas | 0.086 | 0.470 |
| Rpim | 0.026 | 0.138 |
| Total number of observations | 325806 | 23609 |
| Number of reflections | 29554 | 2110 |
| <I/σ(I)> | 27.3 | 5.4 |
| Completeness [%] | 99.0 | |
| Redundancy | 11 | 11.2 |
| CC(1/2) | 0.999 | 0.954 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | 0.2 M Magnesium nitrate hexahydrate, 20% w/v Polyethylene glycol 3,350 |
