8ZB4

Crystal structure of NudC from Mycobacterium abscessus in complex with NAD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-24
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9792
Spacegroup name	P 61 2 2
Unit cell lengths	132.726, 132.726, 175.356
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.700
R-factor	0.22407
Rwork	0.222
R-free	0.26636
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.343
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.830
High resolution limit [Å]	2.700	2.700
Rmerge	0.084	0.908
Rmeas	0.087	0.947
Rpim	0.023	0.264
Total number of observations	365360	42020
Number of reflections	25670	3354
<I/σ(I)>	24	2.9
Completeness [%]	99.8
Redundancy	14.2	12.5
CC(1/2)	0.999	0.878

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	10% PEG8000, 0.1 M imidazole pH 8.0, 0.2 M Ca(OAc)2