8ZB2

L-Methionine oxidase from Burkholderiales bacterium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 65
Unit cell lengths	124.788, 124.788, 148.280
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.780 - 2.690
R-factor	0.22343
Rwork	0.220
R-free	0.28316
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.618
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.784	2.840
High resolution limit [Å]	2.690	2.690
Rmerge	0.063	1.313
Number of reflections	36298	5286
<I/σ(I)>	28.5	2.1
Completeness [%]	100.0
Redundancy	10.3
CC(1/2)	1.000	0.807

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	20% (v/v) PEG 3350, 0.2 M Potassium nitrate, and 0.2 M potassium thiocyanate