8ZAK

Crystal structure of the channel protein CorA from Campylobacter jejuni in complex with Ni2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-02
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9793
Spacegroup name	P 43 21 2
Unit cell lengths	95.742, 95.742, 110.905
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.260 - 2.400
R-factor	0.2012
Rwork	0.199
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.831
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.440
High resolution limit [Å]	2.400	2.400
Number of reflections	20759	1010
<I/σ(I)>	16.8	1.6
Completeness [%]	99.8	99.3
Redundancy	7.9	6.9
CC(1/2)	1.000	0.710

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	PEG 400, lithium sulfate, Tris