8ZAH

Crystal structure of the channel protein CorA from Campylobacter jejuni

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2022-12-05
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 43 21 2
Unit cell lengths	95.620, 95.620, 110.304
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.160 - 2.100
R-factor	0.214
Rwork	0.212
R-free	0.24820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.886
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.140
High resolution limit [Å]	2.100	2.100
Number of reflections	30273	1476
<I/σ(I)>	13.7	1.6
Completeness [%]	98.9	98.4
Redundancy	4.7	4.9
CC(1/2)	0.991	0.657

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	PEG 400, lithium sulfate, Hepes