8ZA7
ART-ARH type toxin effector-immunity protein pair of Listeria monocytogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 31 |
| Unit cell lengths | 84.020, 84.020, 82.810 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.490 - 1.650 |
| R-factor | 0.1689 |
| Rwork | 0.168 |
| R-free | 0.19300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.244 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.400 | 1.690 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.104 | |
| Number of reflections | 78696 | 5848 |
| <I/σ(I)> | 9.2 | |
| Completeness [%] | 100.0 | |
| Redundancy | 2.5 | |
| CC(1/2) | 0.995 | 0.651 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | COUNTER-DIFFUSION | 289.15 | 0.2M Magnesium chloride, 0.1M Sodium cacolylate pH 6.5, 20% PEG1000 |
