8ZA5

Crystal structure of HsmR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2022-10-18
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97933
Spacegroup name	P 32 2 1
Unit cell lengths	97.875, 97.875, 56.855
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	84.760 - 2.200
R-factor	0.18259
Rwork	0.178
R-free	0.26421
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.709
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MoRDa
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.760	2.240
High resolution limit [Å]	2.200	2.200
Rmeas	0.114	1.353
Number of reflections	16200	791
<I/σ(I)>	54.1	6.5
Completeness [%]	100.0
Redundancy	10.5
CC(1/2)	1.000	0.914

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.2 M MgCl2, 25 % (w/v) PEG 3350, and 0.1 M Bis-Tris pH 6.5