8ZA4

Crystal structure of OR4K15 ribozyme mutant - chainBDF C2U

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-29
Detector	DECTRIS EIGER2 X 4M
Wavelength(s)	0.979183
Spacegroup name	C 1 2 1
Unit cell lengths	73.170, 37.354, 116.136
Unit cell angles	90.00, 91.88, 90.00

Refinement procedure

Resolution	35.210 - 2.850
R-factor	0.1981
Rwork	0.198
R-free	0.20660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.432
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.210	3.010
High resolution limit [Å]	2.850	2.850
Number of reflections	7477	1069
<I/σ(I)>	7.6
Completeness [%]	99.2
Redundancy	5.7
CC(1/2)	0.992	0.951

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M Sodium Acetate trihydrate 0.1 M Tris Hydrochloride pH pH 5.8 25%w/v Polyethylene Glycol 4000