8ZA0

Crystal structure of OR4K15 ribozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-29
Detector	DECTRIS EIGER2 X 4M
Wavelength(s)	0.978557
Spacegroup name	C 1 2 1
Unit cell lengths	72.082, 36.570, 113.940
Unit cell angles	90.00, 91.29, 90.00

Refinement procedure

Resolution	56.960 - 1.960
R-factor	0.2045
Rwork	0.203
R-free	0.22980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	2.087
Data reduction software	PROCOR
Data scaling software	PROCOR
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.960	2.010
High resolution limit [Å]	1.960	1.960
Number of reflections	21733	1609
<I/σ(I)>	5.9
Completeness [%]	100.0
Redundancy	6.1
CC(1/2)	0.995	0.657

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M Sodium Cacodylate pH 5.6 1.5 M Sodium Acetate trihydrate