8Z92
Crystal structure of CrtAgo/TIR-APAZ in complex with guide DNA and 16-nt target DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97852 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 201.179, 287.266, 111.766 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.250 - 3.850 |
| R-factor | 0.2483 |
| Rwork | 0.247 |
| R-free | 0.26480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.807 |
| Data reduction software | HKL-3000 (7.21) |
| Data scaling software | HKL-3000 (7.21) |
| Phasing software | PHENIX (1.17.1_3660) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.920 |
| High resolution limit [Å] | 3.850 | 3.850 |
| Rpim | 0.170 | 0.518 |
| Number of reflections | 38276 | 1544 |
| <I/σ(I)> | 3.5 | 1.33 |
| Completeness [%] | 99.6 | 99.4 |
| Redundancy | 4.6 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1 M BIS-TRIS propane, pH 9.0 and 8% w/v Polyethylene glycol 20000 |
