8Z8Y
Crystal structure of CrtAgo/CrtTIR-APAZ complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-25 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.98322 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 198.360, 198.360, 184.350 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.610 - 3.180 |
| R-factor | 0.2755 |
| Rwork | 0.274 |
| R-free | 0.29930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.646 |
| Data reduction software | HKL-3000 (7.21) |
| Data scaling software | HKL-3000 (1.17.1_3660) |
| Phasing software | PHENIX (1.17.1_3660) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.610 | 3.260 |
| High resolution limit [Å] | 3.180 | 3.180 |
| Rpim | 0.052 | 1.000 |
| Number of reflections | 36475 | 2646 |
| <I/σ(I)> | 12.5 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 39.6 | 40 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 2.0 M Ammonium sulfate, 0.1 M Tris, pH 8.0 |
