8Z8U

Crystal structure of LINE-1 ribozyme mutant - C39U

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-01
Detector	DECTRIS EIGER2 X 4M
Wavelength(s)	0.979137
Spacegroup name	P 61 2 2
Unit cell lengths	94.871, 94.871, 261.635
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.590 - 2.710
R-factor	0.2203
Rwork	0.219
R-free	0.24630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.915
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.870
High resolution limit [Å]	2.710	2.720
Number of reflections	19754	2792
<I/σ(I)>	5.8
Completeness [%]	99.8
Redundancy	5.2
CC(1/2)	0.992	0.464

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.01 M Magnesium Chloride 0.05 M MES pH 5.6 2.0 M Lithium Sulfate monohydrate