8Z7K

Crystal structure of Hemolysin co-regulated protein 1 (Hcp1) VariantB from Burkholderia pseudomallei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2021-08-13
Detector	RAYONIX MX300-HS
Wavelength(s)	0.999840
Spacegroup name	P 6
Unit cell lengths	82.721, 82.721, 64.066
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.760 - 1.582
Rwork	0.225
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.664
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.760	47.760	1.610
High resolution limit [Å]	1.580	8.660	1.580
Rmerge	0.044	0.026	2.013
Rmeas	0.048	0.029	2.503
Rpim	0.021	0.013	1.458
Number of reflections	32755	226	1211
<I/σ(I)>	22.8	74.3	0.7
Completeness [%]	95.9	97.8	71.3
Redundancy	9.8	9.5	4.4
CC(1/2)	1.000	0.999	0.258

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	1.5 M ammonium sulfate, 3.75% v/v 2-propanol, 25% glycerol