Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-17 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.815, 73.956, 77.074 |
| Unit cell angles | 90.00, 93.95, 90.00 |
Refinement procedure
| Resolution | 44.192 - 1.545 |
| R-factor | 0.1684 |
| Rwork | 0.167 |
| R-free | 0.19490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.018 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1690) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.580 |
| High resolution limit [Å] | 1.545 | 4.210 | 1.550 |
| Rmerge | 0.097 | 0.075 | 0.646 |
| Rmeas | 0.113 | 0.087 | 0.760 |
| Rpim | 0.058 | 0.045 | 0.397 |
| Number of reflections | 92904 | 4788 | 4640 |
| <I/σ(I)> | 9.9 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 3.8 | 3.8 | 3.7 |
| CC(1/2) | 0.973 | 0.694 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Bis-Tris propane (pH~7.5), 0.2M Sodium Formate or Fluoride, 10% Ethylene Glycol, ~25% PEG3350 |
