8Z7A

Open Barrel Structure of Translin from Trichoderma virens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-02-06
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	76.552, 153.223, 103.949
Unit cell angles	90.00, 111.47, 90.00

Refinement procedure

Resolution	38.280 - 3.200
R-factor	0.2141
Rwork	0.213
R-free	0.23690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.512
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.1_4122: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.510	3.340
High resolution limit [Å]	3.200	3.200
Rmerge	0.072	0.496
Rmeas	0.084	0.576
Rpim	0.043	0.293
Total number of observations	141391
Number of reflections	36769	4492
<I/σ(I)>	10.9	2.1
Completeness [%]	99.9
Redundancy	3.8	3.9
CC(1/2)	0.999	0.841

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	18% PEG 1450, 0.18M Magnesium Chloride, 0.1M sodium cacodylate pH 6.8