8Z79

Crystal structure of 5-N-alpha-glycinylthymidine (N-alpha-GlyT) FAD-dependent lyase gp47/NGTO from Pseudomonas phage PaMx11 in complex with dsDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97852
Spacegroup name	P 1 21 1
Unit cell lengths	79.380, 102.770, 89.068
Unit cell angles	90.00, 107.98, 90.00

Refinement procedure

Resolution	29.760 - 2.650
R-factor	0.2108
Rwork	0.209
R-free	0.24640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8z2o
RMSD bond length	0.009
RMSD bond angle	1.264
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.21rc1_5156)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.790
High resolution limit [Å]	2.650	2.650
Rmerge	0.118	0.779
Rmeas	0.133	0.872
Rpim	0.060	0.383
Number of reflections	39566	5746
<I/σ(I)>	9.7	2
Completeness [%]	99.8	100
Redundancy	4.7	4.8
CC(1/2)	0.997	0.785

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% (w/v) PEG 4,000, 0.1M Sodium citrate/Citric acid pH 5.5, 10% (v/v) 2-propanol