8Z63

alpha-L-fucosidase from Pontiella sulfatireligans F21

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.978
Spacegroup name	P 1 21 1
Unit cell lengths	52.061, 79.822, 89.724
Unit cell angles	90.00, 98.85, 90.00

Refinement procedure

Resolution	47.800 - 2.820
R-factor	0.2194
Rwork	0.216
R-free	0.27540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.933
Data reduction software	HKL-2000
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.800	2.922
High resolution limit [Å]	2.820	2.822
Rmerge	0.110
Number of reflections	16715	16715
<I/σ(I)>	12.27
Completeness [%]	94.8
Redundancy	6.5
CC(1/2)	0.997	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	PEG 4000, Sodium acetate trihydrate