8Z58

Crystal structure of human N141V-SIRT5 in complex with succinylated Prx1 fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	43.916, 73.430, 163.774
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.690 - 2.405
R-factor	0.2145
Rwork	0.212
R-free	0.25820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.720
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.690	2.490
High resolution limit [Å]	2.400	2.400
Rmeas	0.129	0.652
Number of reflections	10588	1034
<I/σ(I)>	10.1	3.2
Completeness [%]	98.9
Redundancy	5.98
CC(1/2)	0.995	0.899

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	24% PEG 3350, 0.1M Tris-HCl pH 8.5