8Z56

Crystal structure of human Q140L-SIRT5 in complex with succinylated Prx1 fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	46.117, 73.056, 164.758
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.997 - 1.806
R-factor	0.2107
Rwork	0.209
R-free	0.23360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.809
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.190	1.870
High resolution limit [Å]	1.806	1.810
Rmeas	0.061	0.849
Number of reflections	25948	2510
<I/σ(I)>	26	3.3
Completeness [%]	99.7
Redundancy	13.41
CC(1/2)	1.000	0.947

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	24% PEG 3350, 0.1M Tris-HCl pH 8.5