8Z4F

Pseudomurein Endoisopeptidases PeiP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	I 2 2 2
Unit cell lengths	105.340, 113.100, 120.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.640 - 1.880
R-factor	0.1974
Rwork	0.196
R-free	0.23110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.141
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.640	1.947
High resolution limit [Å]	1.880	1.880
Rmeas	0.028
Number of reflections	54666	54666
<I/σ(I)>	22.13
Completeness [%]	92.8
Redundancy	3.3
CC(1/2)	1.000	0.637

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	Precipitant:1.00 M Ammonium sulfate; Buffer:100 mM Sodium acetate; pH 4.6;