8Z4C

The Fab fragment of anti-Fibrin monoclonal antibody 59D8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-01-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9786
Spacegroup name	P 1 21 1
Unit cell lengths	71.160, 65.290, 100.140
Unit cell angles	90.00, 97.90, 90.00

Refinement procedure

Resolution	43.500 - 2.060
R-factor	0.1994
Rwork	0.198
R-free	0.23390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.806
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.500	2.130
High resolution limit [Å]	2.060	2.060
Rmerge	0.060	0.352
Number of reflections	56469	5118
<I/σ(I)>	8.4
Completeness [%]	99.9
Redundancy	2
CC(1/2)	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	100 mM Citric Acid (pH 5.0), 1 M Lithium chloride, 10% w/v polyethylene glycol 6000