8Z48
Beta-galactosidase from Bacteroides xylanisolvens (complex with methyl beta-galactopyranose)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-29 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 231.765, 50.839, 229.445 |
| Unit cell angles | 90.00, 96.10, 90.00 |
Refinement procedure
| Resolution | 45.629 - 1.940 |
| Rwork | 0.211 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.535 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.910 | 1.970 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Number of reflections | 194503 | 9674 |
| <I/σ(I)> | 8.1 | 2 |
| Completeness [%] | 98.2 | |
| Redundancy | 3.1 | |
| CC(1/2) | 0.997 | 0.768 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | potassium thiocyanate, PEG 3350 |
