8Z47

Beta-galactosidase from Bacteroides xylanisolvens (ligand-free)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	I 4
Unit cell lengths	163.831, 163.831, 50.857
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.779 - 1.860
Rwork	0.193
R-free	0.22650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.684
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.780	1.900
High resolution limit [Å]	1.860	1.860
Number of reflections	57035	3503
<I/σ(I)>	10.3	2.2
Completeness [%]	100.0
Redundancy	6.6
CC(1/2)	0.996	0.711

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	potassium thiocyanate, PEG 3350