8Z43

Beta-galactosidase from Bacteroides xylanisolvens (E350G, ligand-free)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	I 1 2 1
Unit cell lengths	144.699, 51.062, 180.085
Unit cell angles	90.00, 90.42, 90.00

Refinement procedure

Resolution	48.152 - 1.910
Rwork	0.174
R-free	0.20200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.682
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.198	1.940
High resolution limit [Å]	1.910	1.910
Number of reflections	102654	5058
<I/σ(I)>	15.8	2.2
Completeness [%]	99.9
Redundancy	6.6
CC(1/2)	0.999	0.754

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	sodium citrate tribasic dihydrate, PEG 3350, bis-Tris propane