8Z3I

Crystal Structure of Protein Glutaminase FBPG(Flavobacterium sp.316)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-01-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	51.338, 52.455, 109.467
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.263 - 1.880
R-factor	0.165299339158
Rwork	0.161
R-free	0.20761
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.895
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.910
High resolution limit [Å]	1.880	1.880
Number of reflections	24837	24837
<I/σ(I)>	1.67
Completeness [%]	100.0
Redundancy	11.5
CC(1/2)	1.000	0.844

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	289.17	0.1 M HEPES pH 7.4, 25% (w/v)polyethylene glycol 3350