8Z2Y

High-resolution crystal structure of exo-beta-(1,3)-glucanase from Aspergillus oryzae (AoBgl) as a complex with glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	100
Detector technology	CCD
Collection date	2024-02-27
Detector	MAR CCD 300 mm
Wavelength(s)	0.97893
Spacegroup name	P 32 2 1
Unit cell lengths	81.290, 81.290, 114.740
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.530 - 1.200
R-factor	0.1622
Rwork	0.161
R-free	0.18505
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.791
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0411)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.400
High resolution limit [Å]	1.200	1.200
Number of reflections	134619	48574
<I/σ(I)>	13.72
Completeness [%]	98.3
Redundancy	10.17
CC(1/2)	0.999	0.858

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	291	0.2M Sodium tartrate dibasic dihydrate, 20% w/v Polyethylene glycol 3350