8Z2R
Crystal structure of trehalose synthase mutant N253T from Deinococcus radiodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-01 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.599, 195.382, 131.317 |
| Unit cell angles | 90.00, 92.82, 90.00 |
Refinement procedure
| Resolution | 27.790 - 2.530 |
| R-factor | 0.2207 |
| Rwork | 0.217 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.105 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.530 | 2.530 |
| Rmerge | 0.161 | 0.220 |
| Number of reflections | 146481 | 146481 |
| <I/σ(I)> | 5.2 | |
| Completeness [%] | 99.8 | |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Tris-HCl pH 8.5, 8% (w/v) polyethylene glycol (PEG) 8000 |
