8Z2O

Crystal structure of 5-N-alpha-glycinylthymidine (N-alpha-GlyT) FAD-dependent lyase gp47/NGTO from Pseudomonads phage PaMx11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97852
Spacegroup name	P 32 2 1
Unit cell lengths	86.555, 86.555, 146.699
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.750 - 2.300
R-factor	0.1886
Rwork	0.188
R-free	0.21130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.923
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.126	1.201
Rmeas	0.130	1.236
Rpim	0.029	0.288
Number of reflections	28939	4153
<I/σ(I)>	21.4	3
Completeness [%]	99.9	100
Redundancy	19.6	18.1
CC(1/2)	0.999	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.0 M Potassium/sodium tartrate, 0.1M CHES/Sodium hydroxide pH 9.5, and 0.2M Lithium sulfate