8Z2L
Crystal structure of trehalose synthase mutant N253E from Deinococcus radiodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-04-10 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 96.261, 132.452, 197.294 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.470 - 3.040 |
| R-factor | 0.1653 |
| Rwork | 0.161 |
| R-free | 0.24080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.188 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.170 |
| High resolution limit [Å] | 3.040 | 3.040 |
| Rmerge | 0.229 | 0.991 |
| Number of reflections | 47172 | 24560 |
| <I/σ(I)> | 8.3 | |
| Completeness [%] | 98.5 | |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Tris-HCl pH 8.5, and 8% (w/v) polyethylene glycol (PEG) 8000 |
