8Z29

Crystal structure of apo Aspergillus terreus glutamate dehydrogenase (AtGDH) deletion mutant (T262-A263)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-02-21
Detector	RIGAKU
Wavelength(s)	1.5418
Spacegroup name	P 63
Unit cell lengths	157.550, 157.550, 106.280
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.850
Rwork	0.190
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xvi
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	BALBES
Refinement software	PHENIX (v1.20.1-4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.950
High resolution limit [Å]	2.850	2.850
Number of reflections	35108	3406
<I/σ(I)>	10.5
Completeness [%]	99.9
Redundancy	10.4
CC(1/2)	0.980	0.560

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	15% PEG3350, 0.1 M HEPES pH 7.0, 0.2 M NaCl